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KMID : 1005220090240040253
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Journal of Embryo Transfer
2009 Volume.24 No. 4 p.253 ~ p.257
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Post-Thaw Cryosurvival of Bovine Embryos Produced In Vitro and In Vivo after Controlled Freezing
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Cho Sang-Rae
Choe Chang-Yong
Son Dong-Soo
Kim Hyun-Jong
Kim Jae-Bum
Choi Sun-Ho
Lee Poong-Yeon
Kim Sung-Jae
Son Jun-Kyu
Shin Seung-Oh
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Abstract
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To enhance the embryo preservation technology and better application of embryo transfer technique to the field (dairy science or animal reproduction. etc.), we examined the viabilities of bovine embryos produced in vitro and in vivo after cryopreservation according to their developmental stage and thawing temperature. Bovine embryos from in vivo/vitro fertilization (Hanwoo) were examined at day 7, 8, and 9. Survival rates and total cell numbers of in vivo fertilized embryos were as follows: morulae 68.8% and 67 ± 6.0; blastocysts 80.5% and 120 ± 10; expanded blastocysts 77.4% and 138 ± 9.7, respectively. Rates of embryo development for blastocysts and expanded blastocysts after thawing were significantly higher than that of morula stage embryos (p<0.05). While survival rates of in vitro fertilized embryos according to developmental stage showed no significant difference among groups (morula 67.9%; blastocyst 74.3%; and expanded blastocyst 79.4%), total cell numbers were significantly lower than those of other groups (morula 64 ± 5.9; blastocyst 116 ± 8.7; and expanded blastocyst 135 ± 9.1) For the viability according to thawing temperature, survival rate was higher in 37℃.
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KEYWORD
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bovine, embryo, cryopreservation, in vivo, in vitro, thawing
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